After the induction of apoptosis in the Burkitt's lymphoma cell line BL602, HeLa cells or the T cell line H9 by various stimuli, MCM proteins and Cdc6 were analyzed by immunoblotting. Proteolytic cleavage of Mcm3 and Cdc6 was observed in all cases (Figure 1a, upper and middle panel), while Mcm2 and Mcm4 remained unchanged (data not shown). The http://www.mcmcity.com/ - MCM MCM 財布 http://www.mcmcity.com/ observed Mcm3 fragment has an apparent size of 90 kDa in agreement with previous reports.7, 8 The main cleavage product of Cdc6 had an apparent size of 45 http://www.mcmyume.com/ - MCM MCM バッグ http://www.mcmyume.com/ kDa with a second fragment of 35 kDa appearing later in the apoptotic process (Figure http://www.mcmnolife.com/ - MCM リュック MCM バッグ http://www.mcmnolife.com/ 1a, middle panel).
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